A Coculture Based Tyrosine-Tyrosinase Electrochemical Gene Circuit for Connecting Cellular Communication with Electronic Networks.

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TitleA Coculture Based Tyrosine-Tyrosinase Electrochemical Gene Circuit for Connecting Cellular Communication with Electronic Networks.
Publication TypeJournal Article
Year of Publication2020
AuthorsVanArsdale, E, Hörnström, D, Sjöberg, G, Järbur, I, Pitzer, J, Payne, GF, van Maris, AJA, Bentley, WE
JournalACS Synth Biol
Date Published2020 Apr 02
ISSN2161-5063
Abstract

There is a growing interest in mediating information transfer between biology and electronics. By the addition of redox mediators to various samples and cells, one can both electronically obtain a redox "portrait" of a biological system and, conversely, program gene expression. Here, we have created a cell-based synthetic biology-electrochemical axis in which engineered cells process molecular cues, producing an output that can be directly recorded via electronics-but without the need for added redox mediators. The process is robust; two key components must act together to provide a valid signal. The system builds on the tyrosinase-mediated conversion of tyrosine to L-DOPA and L-DOPAquinone, which are both redox active. "Catalytic" transducer cells provide for signal-mediated surface expression of tyrosinase. Additionally, "reagent" transducer cells synthesize and export tyrosine, a substrate for tyrosinase. In cocultures, this system enables real-time electrochemical transduction of cell activating molecular cues. To demonstrate, we eavesdrop on quorum sensing signaling molecules that are secreted by , -(3-oxododecanoyl)-l-homoserine lactone and pyocyanin.

DOI10.1021/acssynbio.9b00469
Alternate JournalACS Synth Biol
PubMed ID32208720