A redox-based electrogenetic CRISPR system to connect with and control biological information networks.

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TitleA redox-based electrogenetic CRISPR system to connect with and control biological information networks.
Publication TypeJournal Article
Year of Publication2020
AuthorsBhokisham, N, VanArsdale, E, Stephens, KT, Hauk, P, Payne, GF, Bentley, WE
JournalNat Commun
Volume11
Issue1
Pagination2427
Date Published2020 May 15
ISSN2041-1723
Abstract

Electronic information can be transmitted to cells directly from microelectronics via electrode-activated redox mediators. These transmissions are decoded by redox-responsive promoters which enable user-specified control over biological function. Here, we build on this redox communication modality by establishing an electronic eCRISPR conduit of information exchange. This system acts as a biological signal processor, amplifying signal reception and filtering biological noise. We electronically amplify bacterial quorum sensing (QS) signaling by activating LasI, the autoinducer-1 synthase. Similarly, we filter out unintended noise by inhibiting the native SoxRS-mediated oxidative stress response regulon. We then construct an eCRISPR based redox conduit in both E. coli and Salmonella enterica. Finally, we display eCRISPR based information processing that allows transmission of spatiotemporal redox commands which are then decoded by gelatin-encapsulated E. coli. We anticipate that redox communication channels will enable biohybrid microelectronic devices that could transform our abilities to electronically interpret and control biological function.

DOI10.1038/s41467-020-16249-x
Alternate JournalNat Commun
PubMed ID32415193
PubMed Central IDPMC7228920
Grant ListR21EB024102 / / U.S. Department of Health & Human Services | National Institutes of Health (NIH) /
CBET 1805274 / / National Science Foundation (NSF) /
ECCS 1807604 / / National Science Foundation (NSF) /
HDTRA1-19-0021 / / United States Department of Defense | Defense Threat Reduction Agency (DTRA) /