Dynamic changes impact the plum pox virus population structure during leaf and bud development.

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TitleDynamic changes impact the plum pox virus population structure during leaf and bud development.
Publication TypeJournal Article
Year of Publication2020
AuthorsTamukong, YB, Collum, TD, Stone, AL, Kappagantu, M, Sherman, DJ, Rogers, EE, Dardick, C, Culver, JN
JournalVirology
Volume548
Pagination192-199
Date Published2020 09
ISSN1096-0341
KeywordsFruit, Genome, Viral, Phloem, Plant Diseases, Plant Leaves, Plum Pox Virus, Prunus domestica
Abstract

Plum pox virus (PPV) is a worldwide threat to stone fruit production. Its woody perennial hosts provide a dynamic environment for virus evolution over multiple growing seasons. To investigate the impact seasonal host development plays in PPV population structure, next generation sequencing of ribosome associated viral genomes, termed translatome, was used to assess PPV variants derived from phloem or whole leaf tissues over a range of plum leaf and bud developmental stages. Results show that translatome PPV variants occur at proportionately higher levels in bud and newly developing leaf tissues that have low infection levels while more mature tissues with high infection levels display proportionately lower numbers of viral variants. Additional variant analysis identified distinct groups based on population frequency as well as sets of phloem and whole tissue specific variants. Combined, these results indicate PPV population dynamics are impacted by the tissue type and developmental stage of their host.

DOI10.1016/j.virol.2020.06.014
Alternate JournalVirology
PubMed ID32758716
PubMed Central IDPMC7513809
Grant ListT32 AI051967 / AI / NIAID NIH HHS / United States