RPW8.1 enhances the ethylene-signaling pathway to feedback-attenuate its mediated cell death and disease resistance in Arabidopsis.

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TitleRPW8.1 enhances the ethylene-signaling pathway to feedback-attenuate its mediated cell death and disease resistance in Arabidopsis.
Publication TypeJournal Article
Year of Publication2020
AuthorsZhao, Z-X, Feng, Q, Liu, P-Q, He, X-R, Zhao, J-H, Xu, Y-J, Zhang, L-L, Huang, Y-Y, Zhao, J-Q, Fan, J, Li, Y, Xiao, S, Wang, W-M
JournalNew Phytol
Date Published2020 Aug 07
ISSN1469-8137
Abstract

The Arabidopsis RESISTANCE TO POWDERY MILDEW 8.1 (RPW8.1) activates confined cell death and defense against different pathogens. However, the underlying regulatory mechanisms still remain elusive. Here, we show that RPW8.1 activates ethylene signaling that, in turn, negatively regulates RPW8.1 expression. RPW8.1 binds and stabilizes 1-aminocyclopropane-1-carboxylate oxidase 4 (ACO4), which may in part explain increased ethylene production and signaling in RPW8.1-expressing plants. In return, ACO4 and other key components of ethylene signaling negatively regulate RPW8.1-mediated cell death and disease resistance via suppressing RPW8.1 expression. Loss of function in ACO4, EIN2, EIN3 EIL1, ERF6, ERF016 or ORA59 increases RPW8.1-mediated cell death and defense response. By contrast, overexpression of EIN3 abolishes or significantly compromises RPW8.1-mediated cell death and disease resistance. Furthermore, ERF6, ERF016 and ORA59 appear to act as trans-repressors of RPW8.1, with OAR59 being able to directly bind to the RPW8.1 promoter. Taken together, our results have revealed a feedback regulatory circuit connecting RPW8.1 and the ethylene-signaling pathway, in which RPW8.1 enhances ethylene signaling, and the latter, in return, negatively regulates RPW8.1-mediated cell death and defense response via suppressing RPW8.1 expression to attenuate its defense activity.

DOI10.1111/nph.16857
Alternate JournalNew Phytol
PubMed ID32767839
Grant List31371931 / / National Natural Science Foundation of China /
31430072 / / National Natural Science Foundation of China /
31672090 / / National Natural Science Foundation of China /
IOS-1457033 / / Natural Science Foundation /
IOS-1901566 / / Natural Science Foundation /
/ / China Scholarship Council /