Bioelectronic control of a microbial community using surface-assembled electrogenetic cells to route signals.

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TitleBioelectronic control of a microbial community using surface-assembled electrogenetic cells to route signals.
Publication TypeJournal Article
Year of Publication2021
AuthorsTerrell, JL, Tschirhart, T, Jahnke, JP, Stephens, K, Liu, Y, Dong, H, Hurley, MM, Pozo, M, Mckay, R, Tsao, CYu, Wu, H-C, Vora, G, Payne, GF, Stratis-Cullum, DN, Bentley, WE
JournalNat Nanotechnol
Date Published2021 Mar 29
ISSN1748-3395
Abstract

We developed a bioelectronic communication system that is enabled by a redox signal transduction modality to exchange information between a living cell-embedded bioelectronics interface and an engineered microbial network. A naturally communicating three-member microbial network is 'plugged into' an external electronic system that interrogates and controls biological function in real time. First, electrode-generated redox molecules are programmed to activate gene expression in an engineered population of electrode-attached bacterial cells, effectively creating a living transducer electrode. These cells interpret and translate electronic signals and then transmit this information biologically by producing quorum sensing molecules that are, in turn, interpreted by a planktonic coculture. The propagated molecular communication drives expression and secretion of a therapeutic peptide from one strain and simultaneously enables direct electronic feedback from the second strain, thus enabling real-time electronic verification of biological signal propagation. Overall, we show how this multifunctional bioelectronic platform, termed a BioLAN, reliably facilitates on-demand bioelectronic communication and concurrently performs programmed tasks.

DOI10.1038/s41565-021-00878-4
Alternate JournalNat Nanotechnol
PubMed ID33782589
Grant ListHDTRA1-19-0021 / / United States Department of Defense | Defense Threat Reduction Agency (DTRA) /
DMREF1435957 / / NSF | Directorate for Mathematical & Physical Sciences | Division of Materials Research (DMR) /
CBET1805274 / / NSF | ENG/OAD | Division of Chemical, Bioengineering, Environmental, and Transport Systems (CBET) /
ECCS1807604 / / NSF | ENG/OAD | Division of Electrical, Communications and Cyber Systems (ECCS) /
R21EB024102 / / U.S. Department of Health & Human Services | NIH | National Institute of Biomedical Imaging and Bioengineering (NIBIB) /
N0001417WX01318 / / United States Department of Defense | United States Navy | Office of Naval Research (ONR) /
N0001418WX01042 / / United States Department of Defense | United States Navy | Office of Naval Research (ONR) /