Genome Sequence Resource for Erysiphe necator NAFU1, a Grapevine Powdery Mildew Isolate Identified in Shaanxi Province of China.

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TitleGenome Sequence Resource for Erysiphe necator NAFU1, a Grapevine Powdery Mildew Isolate Identified in Shaanxi Province of China.
Publication TypeJournal Article
Year of Publication2021
AuthorsZhang, X, Mu, B, Cui, K, Liu, M, Ke, G, Han, Y, Wu, Y, Xiao, S, Wen, Y
JournalMol Plant Microbe Interact
Date Published2021 Aug 26
ISSN0894-0282
Abstract

Erysiphe necator is an economically important biotrophic fungal pathogen responsible for powdery mildew disease on grapevine. Currently, genome sequences are available for only a few Erysiphe necator isolates from USA. Based on the combination of Nanopore and Illumina sequencing technologies, we present here the complete genome assembly for an isolate of E. necator NAFU1 identified in China. We acquired a total of 15.93 Gb raw reads. These reads were processed into a 61.12 Mb genome assembly containing 73 contigs with the N50 of 2.06 Mb and a maximum length of 6.05 Mb. Combining the results of three gene-prediction modules, i.e. an evidence-based gene modeler (EVidenceModeler or EVM), an ab initio gene modeler, and a homology-based gene modeler, we predicted 7235 protein-coding genes in the assembled genome of E. necator NAFU1. This information will facilitate studies of genome evolution and pathogenicity mechanisms of E. necator and other powdery mildew species through comparative genome sequence analysis and other molecular genetic tools.

DOI10.1094/MPMI-03-21-0061-A
Alternate JournalMol Plant Microbe Interact
PubMed ID34445887