Oxygen microscopy by two-photon-excited phosphorescence.

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TitleOxygen microscopy by two-photon-excited phosphorescence.
Publication TypeJournal Article
Year of Publication2008
AuthorsFinikova, OS, Lebedev, AY, Aprelev, A, Troxler, T, Gao, F, Garnacho, C, Muro, S, Hochstrasser, RM, Vinogradov, SA
JournalChemphyschem
Volume9
Issue12
Pagination1673-9
Date Published2008 Aug 25
ISSN1439-7641
KeywordsCells, Cultured, Endothelial Cells, Humans, Luminescent Measurements, Microscopy, Molecular Structure, Nanostructures, Oxygen, Photochemistry, Photons
Abstract

High-resolution images of oxygen distributions in microheterogeneous samples are obtained by two-photon laser scanning microscopy (2P LSM), using a newly developed dendritic nanoprobe with internally enhanced two-photon absorption (2PA) cross-section. In this probe, energy is harvested by a 2PA antenna, which passes excitation onto a phosphorescent metalloporphyrin via intramolecular energy transfer. The 2P LSM allows sectioning of oxygen gradients with near diffraction-limited resolution, and lifetime-based acquisition eliminates dependence on the local probe concentration. The technique is validated on objects with a priori known oxygen distributions and applied to imaging of pO(2) in cells.

DOI10.1002/cphc.200800296
Alternate JournalChemphyschem
PubMed ID18663708
PubMed Central IDPMC2645351
Grant ListEB007279 / EB / NIBIB NIH HHS / United States
HL081273 / HL / NHLBI NIH HHS / United States
P41-RR001348 / RR / NCRR NIH HHS / United States
R01 EB007279-02 / EB / NIBIB NIH HHS / United States