Topi, an IS630/Tc1/mariner-type transposable element in the African malaria mosquito, Anopheles gambiae.

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TitleTopi, an IS630/Tc1/mariner-type transposable element in the African malaria mosquito, Anopheles gambiae.
Publication TypeJournal Article
Year of Publication2008
AuthorsSubramanian, RA, Akala, OO, Adejinmi, JO, O'Brochta, DA
JournalGene
Volume423
Issue1
Pagination63-71
Date Published2008 Oct 15
ISSN0378-1119
KeywordsAfrica, Animals, Anopheles gambiae, Base Sequence, DNA Transposable Elements, Gene Dosage, Genes, Insect, Genetic Drift, Open Reading Frames, Phylogeny, Selection, Genetic
Abstract

IS630/Tc1/mariner elements are diverse and widespread within insects. The African malaria mosquito, Anopheles gambiae, contains over 30 families of IS630/Tc1/mariner elements although few have been studied in any detail. To examine the history of Topi elements in An. gambiae populations, Topi elements (n=73) were sampled from five distinct populations of An. gambiae from eastern and western Africa and evaluated with respect to copy number, nucleotide diversity and insertion site-occupancy frequency. Topi 1 and 2 elements were abundant (10-34 per diploid genome) and highly diverse (pi=0.051). Elements from mosquitoes collected in Nigeria were Topi 2 elements and those from mosquitoes collected in Mozambique were Topi 1 elements. Of the 49 Topi transposase open reading frames sequenced none were found to be identical. Intact elements with complete transposase open reading frames were common, although based on insertion site-occupancy frequency data it appeared that genetic drift was the major force acting on these IS630/Tc1/mariner-type elements. Topi 3 elements were not recovered from any of the populations sampled in this study and appear to be rare elements in An. gambiae, possibly due to a recent introduction.

DOI10.1016/j.gene.2008.06.023
Alternate JournalGene
PubMed ID18634859
PubMed Central IDPMC2614661
Grant ListGM48102 / GM / NIGMS NIH HHS / United States
R01 AI070812-03 / AI / NIAID NIH HHS / United States
R01 GM048102-15 / GM / NIGMS NIH HHS / United States