Topi, an IS630/Tc1/mariner-type transposable element in the African malaria mosquito, Anopheles gambiae.

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TitleTopi, an IS630/Tc1/mariner-type transposable element in the African malaria mosquito, Anopheles gambiae.
Publication TypeJournal Article
Year of Publication2008
AuthorsSubramanian, RA, Akala, OO, Adejinmi, JO, O'Brochta, DA
Date Published2008 Oct 15
KeywordsAfrica, Animals, Anopheles gambiae, Base Sequence, DNA Transposable Elements, Gene Dosage, Genes, Insect, Genetic Drift, Open Reading Frames, Phylogeny, Selection, Genetic

IS630/Tc1/mariner elements are diverse and widespread within insects. The African malaria mosquito, Anopheles gambiae, contains over 30 families of IS630/Tc1/mariner elements although few have been studied in any detail. To examine the history of Topi elements in An. gambiae populations, Topi elements (n=73) were sampled from five distinct populations of An. gambiae from eastern and western Africa and evaluated with respect to copy number, nucleotide diversity and insertion site-occupancy frequency. Topi 1 and 2 elements were abundant (10-34 per diploid genome) and highly diverse (pi=0.051). Elements from mosquitoes collected in Nigeria were Topi 2 elements and those from mosquitoes collected in Mozambique were Topi 1 elements. Of the 49 Topi transposase open reading frames sequenced none were found to be identical. Intact elements with complete transposase open reading frames were common, although based on insertion site-occupancy frequency data it appeared that genetic drift was the major force acting on these IS630/Tc1/mariner-type elements. Topi 3 elements were not recovered from any of the populations sampled in this study and appear to be rare elements in An. gambiae, possibly due to a recent introduction.

Alternate JournalGene
PubMed ID18634859
PubMed Central IDPMC2614661
Grant ListGM48102 / GM / NIGMS NIH HHS / United States
R01 AI070812-03 / AI / NIAID NIH HHS / United States
R01 GM048102-15 / GM / NIGMS NIH HHS / United States