Transposable element dynamics of the hAT element Herves in the human malaria vector Anopheles gambiae s.s.

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TitleTransposable element dynamics of the hAT element Herves in the human malaria vector Anopheles gambiae s.s.
Publication TypeJournal Article
Year of Publication2007
AuthorsSubramanian, RA, Arensburger, P, Atkinson, PW, O'Brochta, DA
JournalGenetics
Volume176
Issue4
Pagination2477-87
Date Published2007 Aug
ISSN0016-6731
KeywordsAfrica, Animals, Anopheles gambiae, Base Sequence, DNA Primers, DNA Transposable Elements, Drosophila melanogaster, Genetics, Population, Humans, Insect Vectors, Malaria, Molecular Sequence Data, Open Reading Frames, Polymorphism, Genetic, Selection, Genetic, Species Specificity
Abstract

Transposable elements are being considered as genetic drive agents for introducing phenotype-altering genes into populations of vectors of human disease. The dynamics of endogenous elements will assist in predicting the behavior of introduced elements. Transposable element display was used to estimate the site-occupancy frequency distribution of Herves in six populations of Anopheles gambiae s.s. The site-occupancy distribution data suggest that the element has been recently active within the sampled populations. All 218 individuals sampled contained at least one copy of Herves with a mean of 3.6 elements per diploid genome. No significant differences in copy number were observed among populations. Nucleotide polymorphism within the element was high (pi = 0.0079 in noncoding sequences and 0.0046 in coding sequences) relative to that observed in some of the more well-studied elements in Drosophila melanogaster. In total, 33 distinct forms of Herves were found on the basis of the sequence of the first 528 bp of the transposase open reading frame. Only two forms were found in all six study populations. Although Herves elements in An. gambiae are quite diverse, 85% of the individuals examined had evidence of complete forms of the element. Evidence was found for the lateral transfer of Herves from an unknown source into the An. gambiae lineage prior to the diversification of the An. gambiae species complex. The characteristics of Herves in An. gambiae are somewhat unlike those of P elements in D. melanogaster.

DOI10.1534/genetics.107.071811
Alternate JournalGenetics
PubMed ID17603116
PubMed Central IDPMC1950647
Grant ListR01 GM 48102 / GM / NIGMS NIH HHS / United States