DNA binding by the Methanothermobacter thermautotrophicus Cdc6 protein is inhibited by the minichromosome maintenance helicase.

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TitleDNA binding by the Methanothermobacter thermautotrophicus Cdc6 protein is inhibited by the minichromosome maintenance helicase.
Publication TypeJournal Article
Year of Publication2006
AuthorsKasiviswanathan, R, Shin, J-H, Kelman, Z
JournalJ Bacteriol
Volume188
Issue12
Pagination4577-80
Date Published2006 Jun
ISSN0021-9193
KeywordsArchaeal Proteins, Cell Cycle Proteins, DNA Helicases, DNA Replication, DNA, Archaeal, DNA-Binding Proteins, Methanobacteriaceae, Protein Binding
Abstract

The Cdc6 proteins from the archaeon Methanothermobacter thermautotrophicus were previously shown to bind double-stranded DNA. It is shown here that the proteins also bind single-stranded DNA. Using minichromosome maintenance (MCM) helicase mutant proteins unable to bind DNA, it was found that the interaction of MCM with Cdc6 inhibits the DNA binding activity of Cdc6.

DOI10.1128/JB.00168-06
Alternate JournalJ. Bacteriol.
PubMed ID16740965
PubMed Central IDPMC1482948