Peptidoglycan recognition by Pal, an outer membrane lipoprotein.

Printer-friendly versionPrinter-friendly versionPDF versionPDF version
TitlePeptidoglycan recognition by Pal, an outer membrane lipoprotein.
Publication TypeJournal Article
Year of Publication2006
AuthorsParsons, LM, Lin, F, Orban, J
Date Published2006 Feb 21
KeywordsAmino Acid Sequence, Bacterial Outer Membrane Proteins, Escherichia coli Proteins, Haemophilus influenzae, Influenza Vaccines, Lipoproteins, Molecular Sequence Data, Nuclear Magnetic Resonance, Biomolecular, Peptidoglycan, Sequence Alignment

Peptidoglycan-associated lipoprotein (Pal) is a potential vaccine candidate from Haemophilus influenzae that is highly conserved in Gram-negative bacteria and anchored to the outer membrane through an N-terminal lipid attachment. Pal stabilizes the outer membrane by providing a noncovalent link to the peptidoglycan (PG) layer through a periplasmic domain. Using NMR spectroscopy, we determined the three-dimensional structure of a complex between the periplasmic domain of Pal and a biosynthetic peptidoglycan precursor (PG-P), UDP-N-acetylmuramyl-L-Ala-alpha-d-Glu-m-Dap-D-Ala-d-Ala (m-Dap is meso-diaminopimelate). Pal has a binding pocket lined with conserved surface residues that interacts exclusively with the peptide portion of the ligand. The m-Dap residue, which is mainly found in the cell walls of Gram-negative bacteria, is sequestered in this pocket and plays an important role by forming hydrogen bond and hydrophobic contacts to Pal. The structure provides insight into the mode of cell wall recognition for a broad class of Gram-negative membrane proteins, including OmpA and MotB, which have peptidoglycan-binding domains homologous to that of Pal.

Alternate JournalBiochemistry
PubMed ID16475801
Grant List1S10RR15744 / RR / NCRR NIH HHS / United States
GM57890 / GM / NIGMS NIH HHS / United States