In vivo monitoring of intracellular expression of human interleukin-2 using green fluorescent protein fusion partner in Pichia pastoris.

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TitleIn vivo monitoring of intracellular expression of human interleukin-2 using green fluorescent protein fusion partner in Pichia pastoris.
Publication TypeJournal Article
Year of Publication2004
AuthorsCha, HJoon, Dalal, NG, Bentley, WE
JournalBiotechnol Lett
Volume26
Issue14
Pagination1157-62
Date Published2004 Jul
ISSN0141-5492
KeywordsBiotechnology, Blotting, Western, Dose-Response Relationship, Drug, Escherichia coli, Green Fluorescent Proteins, Humans, Hydrogen-Ion Concentration, Interleukin-2, Pichia, Plasmids, Time Factors
Abstract

The use of Pichia pastoris for protein production was simplified by creation of fusion proteins containing green fluorescent protein (GFP) and the product of interest. Human interleukin-2 (hIL-2) was used as a model product: GFP enabled clear identification of fusion protein expression and, more importantly, the quantification of hIL-2. Although GFP fusions for bioprocess monitoring have been demonstrated in other hosts, this is its first use in P. pastoris.

DOI10.1023/B:BILE.0000035489.09417.d4
Alternate JournalBiotechnol. Lett.
PubMed ID15266123