|Title||In vivo monitoring of intracellular expression of human interleukin-2 using green fluorescent protein fusion partner in Pichia pastoris.|
|Publication Type||Journal Article|
|Year of Publication||2004|
|Authors||Cha, HJoon, Dalal, NG, Bentley, WE|
|Date Published||2004 Jul|
|Keywords||Biotechnology, Blotting, Western, Dose-Response Relationship, Drug, Escherichia coli, Green Fluorescent Proteins, Humans, Hydrogen-Ion Concentration, Interleukin-2, Pichia, Plasmids, Time Factors|
The use of Pichia pastoris for protein production was simplified by creation of fusion proteins containing green fluorescent protein (GFP) and the product of interest. Human interleukin-2 (hIL-2) was used as a model product: GFP enabled clear identification of fusion protein expression and, more importantly, the quantification of hIL-2. Although GFP fusions for bioprocess monitoring have been demonstrated in other hosts, this is its first use in P. pastoris.
|Alternate Journal||Biotechnol. Lett.|