Role of the T cell receptor alpha chain in stabilizing TCR-superantigen-MHC class II complexes.

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TitleRole of the T cell receptor alpha chain in stabilizing TCR-superantigen-MHC class II complexes.
Publication TypeJournal Article
Year of Publication1999
AuthorsAndersen, PS, Lavoie, PM, Sékaly, RP, Churchill, H, Kranz, DM, Schlievert, PM, Karjalainen, K, Mariuzza, RA
JournalImmunity
Volume10
Issue4
Pagination473-83
Date Published1999 Apr
ISSN1074-7613
KeywordsAmino Acid Substitution, Animals, Enterotoxins, HLA-DR1 Antigen, Humans, Macromolecular Substances, Mice, Mice, Knockout, Mice, Transgenic, Models, Molecular, Mutagenesis, Site-Directed, Protein Binding, Receptors, Antigen, T-Cell, alpha-beta, Superantigens, Tumor Cells, Cultured
Abstract

Superantigens (SAGs) activate T cells by simultaneously binding the Vbeta domain of the TCR and MHC class II molecules on antigen-presenting cells. The preferential expression of certain Valpha regions among SAG-reactive T cells has suggested that the TCR alpha chain may modulate the level of activation through an interaction with MHC. We demonstrate that the TCR alpha chain is required for maximum stabilization of the TCR-SAG-MHC complex and that the alpha chain increases the half-life of the complex to match those of TCR-peptide/MHC complexes. The site on the TCR alpha chain responsible for these effects is CDR2. Thus, the overall stability of the TCR-SAG-MHC complex is determined by the combination of three distinct interactions: TCR-SAG, SAG-MHC, and MHC-TCR.

Alternate JournalImmunity
PubMed ID10229190
Grant ListAI36900 / AI / NIAID NIH HHS / United States
AI42937 / AI / NIAID NIH HHS / United States
HL36611 / HL / NHLBI NIH HHS / United States