Escherichia coli expression vectors containing a protein kinase recognition motif, His6-tag and hemagglutinin epitope.

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TitleEscherichia coli expression vectors containing a protein kinase recognition motif, His6-tag and hemagglutinin epitope.
Publication TypeJournal Article
Year of Publication1995
AuthorsKelman, Z, Yao, N, O'Donnell, M
JournalGene
Volume166
Issue1
Pagination177-8
Date Published1995 Dec 1
ISSN0378-1119
KeywordsAmino Acid Sequence, Base Sequence, Cloning, Molecular, Epitopes, Escherichia coli, Gene Expression, Genetic Vectors, Hemagglutinins, Histidine, Molecular Sequence Data, Protein Kinases, Recombinant Fusion Proteins
Abstract

Escherichia coli expression vectors, based on the pET system, were constructed to allow fusion of a protein kinase (PK) recognition motif, a hemagglutinin (HG) epitope-tag and a His6-tag at the N-terminal portion of a protein of interest. The fusion proteins, that result from expression using these vectors, can be phosphorylated in vitro using cAMP-dependent PK, immunoprecipitated using monoclonal antibody against the HG-epitope, and can be rapidly purified using a Ni2+ column.

Alternate JournalGene
PubMed ID8529886
Grant ListGM38839 / GM / NIGMS NIH HHS / United States