Negative regulation of P element excision by the somatic product and terminal sequences of P in Drosophila melanogaster.

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TitleNegative regulation of P element excision by the somatic product and terminal sequences of P in Drosophila melanogaster.
Publication TypeJournal Article
Year of Publication1993
AuthorsHandler, AM, Gomez, SP, O'Brochta, DA
JournalMol Gen Genet
Volume237
Issue1-2
Pagination145-51
Date Published1993 Feb
ISSN0026-8925
KeywordsAnimals, Blotting, Northern, Chromosomes, DNA Transposable Elements, Drosophila melanogaster, Gene Expression Regulation, Enzymologic, Genetic Vectors, Heat-Shock Proteins, Nucleotidyltransferases, Plasmids, Promoter Regions, Genetic, Repressor Proteins, Transcription, Genetic, Transposases
Abstract

A transient in vivo P element excision assay was used to test the regulatory properties of putative repressor-encoding plasmids in Drosophila melanogaster embryos. The somatic expression of an unmodified transposase transcription unit under the control of a heat shock gene promoter (phs pi) effectively repressed P excision in a dose-dependent manner at very low concentrations relative to somatically active transposase (encoded by the hs pi delta 2-3 gene). Maximum repression required transcription of the complete transposase gene. Dose-dependent repression of P excision was also observed in the presence of a vector plasmid (pCarnegie4) having only the terminal sequences, including transposase binding sites, of the P element. However, repression required considerably higher concentrations of pCarnegie4 than phs pi, and elimination of P excision was not observed.

Alternate JournalMol. Gen. Genet.
PubMed ID8384289