Chemical modification of rabbit IgG with N-dansylaziridine. Investigation of the properties of dansylated antibodies.

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TitleChemical modification of rabbit IgG with N-dansylaziridine. Investigation of the properties of dansylated antibodies.
Publication TypeJournal Article
Year of Publication1991
AuthorsPikuleva, IA, Turko, IV, Adamovich, TB, Chashchin, VL
JournalMol Immunol
Volume28
Issue4-5
Pagination311-8
Date Published1991 Apr-May
ISSN0161-5890
KeywordsAmino Acid Sequence, Animals, Antigen-Antibody Reactions, Aziridines, Circular Dichroism, Dansyl Compounds, Immunoglobulin G, Molecular Sequence Data, Pepsin A, Peptide Fragments, Protein Conformation, Rabbits, Spectrometry, Fluorescence, Sulfhydryl Compounds, Thyroglobulin, Trypsin
Abstract

To elucidate the effect of antigen binding fluorescent thiol reagent, N-dansylaziridine (DAZ), which is sensitive to the changes in the microenvironment, was used for modification of rabbit IgG hinge region cysteine residue. DAZ binds to hinge region Cys 226 as evidenced by the structural analysis. Labelling of IgG with DAZ does not alter its conformation, hydrodynamic behavior, nor its antigen binding properties. Upon antigen bindings, the fluorescence intensity of modified IgG increases about 80%. This result indicates that interaction of antibodies with antigen is accompanied by the conformational changes in the IgG hinge region.

Alternate JournalMol. Immunol.
PubMed ID2062314