The induction of respiratory allergy in guinea-pigs following intradermal injection of trimellitic anhydride: a comparison with the response to 2,4-dinitrochlorobenzene.

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TitleThe induction of respiratory allergy in guinea-pigs following intradermal injection of trimellitic anhydride: a comparison with the response to 2,4-dinitrochlorobenzene.
Publication TypeJournal Article
Year of Publication1989
AuthorsBotham, PA, Rattray, NJ, Woodcock, DR, Walsh, STR, Hext, PM
JournalToxicol Lett
Volume47
Issue1
Pagination25-39
Date Published1989 Apr
ISSN0378-4274
KeywordsAdministration, Inhalation, Animals, Antibody Formation, Dinitrochlorobenzene, Epitopes, Female, Guinea Pigs, Haptens, Immunization, Immunoglobulin E, Immunoglobulin G, Injections, Intradermal, Phthalic Acids, Phthalic Anhydrides, Respiratory Hypersensitivity
Abstract

Guinea-pigs injected intradermally with the known respiratory sensitiser trimellitic anhydride (TMA) developed high-titre antigen-specific homocytotropic (IgG1 and IgE) antibodies. Many of the sensitised animals responded to a challenge by inhalation with either free TMA or a TMA-protein conjugate with a change in respiratory rate, reflecting the onset of bronchoconstriction. Guinea-pigs were also injected intradermally with 2,4-dinitrochlorobenzene (DNCB), which is a potent skin sensitiser in man but which has not been reported to cause respiratory allergy. These animals developed only low-titre homocytotropic antibodies and were unresponsive to an inhalation challenge with either free or conjugated hapten. The animals were, however, contact-sensitised to the chemical.

Alternate JournalToxicol. Lett.
PubMed ID2469142