A novel structured kinetic modeling approach for the analysis of plasmid instability in recombinant bacterial cultures.

Printer-friendly versionPrinter-friendly versionPDF versionPDF version
TitleA novel structured kinetic modeling approach for the analysis of plasmid instability in recombinant bacterial cultures.
Publication TypeJournal Article
Year of Publication1989
AuthorsBentley, WE, Kompala, DS
JournalBiotechnol Bioeng
Volume33
Issue1
Pagination49-61
Date Published1989 Jan 5
ISSN0006-3592
Abstract

The instantaneous specific growth rate of a recombinant bacterial culture is directly calculated using a simple structured kinetic modeling approach. Foreign plasmid replication and foreign protein expression represent metabolic burdens to the host cell. The individual effects of these plasmid-mediated activities on the growth rate of plasmid-bearing cells are estimated separately. The dynamic and steady state simulations of the model equations show remarkable agreement with widely observed experimental trends in plasmid copy number and foreign protein content. The model provides an important tool for understanding and controlling plasmid instability in recombinant bacterial fermentations. The modeling framework employed here is suitable for studying the metabolism and growth of a variety of microbial cultures.

DOI10.1002/bit.260330108
Alternate JournalBiotechnol. Bioeng.
PubMed ID18587843