Regulation of expression of the wound tumor virus genome in persistently infected vector cells is related to change in translational activity of viral transcripts.

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TitleRegulation of expression of the wound tumor virus genome in persistently infected vector cells is related to change in translational activity of viral transcripts.
Publication TypeJournal Article
Year of Publication1986
AuthorsPeterson, AJ, Nuss, DL
JournalJ Virol
Volume59
Issue2
Pagination195-202
Date Published1986 Aug
ISSN0022-538X
KeywordsCells, Cultured, Gene Expression Regulation, Genes, Viral, Kinetics, Molecular Weight, Plant Viruses, Protein Biosynthesis, Reoviridae, RNA, Messenger, RNA, Viral, Viral Proteins
Abstract

The interaction between a plant virus and its insect vector was studied at the molecular level by examining wound tumor virus (WTV) gene expression in cultured cells derived from its leafhopper vector. Infection of vector cells by WTV is noncytopathic and results in an acute phase (through day 5), followed by persistence beginning with the first cell passage. Viral-specific polypeptide synthesis and viral genome RNA accumulation increased to a maximum level during the first 5 days following inoculation and then decreased as infected cells were passaged (to 5 to 20% of the level observed during the acute phase by passages 10 to 15). In contrast, viral-specific mRNAs were present at approximately the same level in the acute phase and in the early stage (passage 10) of the persistent phase of infection. Although viral transcripts isolated at different times after inoculation exhibited identical electrophoretic migration patterns, they had different functional activities in cell-free translation systems. Transcripts isolated from persistently infected cells were inefficiently translated in vitro, reflecting the situation in infected cells. These results indicate that the decline in the level of viral polypeptide synthesis associated with the persistent phase of WTV infection is related to a change in the translational activity of viral transcripts.

Alternate JournalJ. Virol.
PubMed ID3735485
PubMed Central IDPMC253066
Grant List1R01-AI 17613 / AI / NIAID NIH HHS / United States