Methyl group analysis of virion-associated high-molecular-weight RNA synthesized in vitro by purified vaccinia virus.

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TitleMethyl group analysis of virion-associated high-molecular-weight RNA synthesized in vitro by purified vaccinia virus.
Publication TypeJournal Article
Year of Publication1977
AuthorsNuss, DL, Paoletti, E
JournalJ Virol
Volume23
Issue1
Pagination110-6
Date Published1977 Jul
ISSN0022-538X
KeywordsBase Sequence, Cell-Free System, HeLa Cells, Methylation, Molecular Weight, RNA, Messenger, RNA, Viral, S-Adenosylmethionine, Uracil Nucleotides, Vaccinia virus
Abstract

The methylation pattern of virion-associated high-molecular-weight RNA synthesized in vitro by purified vaccinia virus has been determined. Analysis of purified high-molecular-weight RNA synthesized with S-[methyl-3H]-adenosylmethionine and alpha[32P]UTP as precursors gave the following results. (i) Eessentially all molecules contained blocked and methylated structures of the type m7G(5')ppp(5')Gm and m7G(5')ppp(5')Am. (ii) There was no detectable methylation at internal sites. (iii) Under several different conditions of synthesis, the ratio of molecules containing m7G(5')ppp(5')Gm to those containing m7G(5')ppp(5')Am was imilar for both the virion-associated high-molecular-weight RNA and the virion-released 8-12S mRNA.

Alternate JournalJ. Virol.
PubMed ID886646
PubMed Central IDPMC515806