Translation of individual host mRNA's in MPC-11 cells is differentially suppressed after infection by vesicular stomatitis virus.

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TitleTranslation of individual host mRNA's in MPC-11 cells is differentially suppressed after infection by vesicular stomatitis virus.
Publication TypeJournal Article
Year of Publication1976
AuthorsNuss, DL, Koch, G
JournalJ Virol
Volume19
Issue2
Pagination572-8
Date Published1976 Aug
ISSN0022-538X
KeywordsAnimals, Cell Line, Immunoglobulin G, Immunoglobulin Heavy Chains, Immunoglobulin Light Chains, Mice, Neoplasm Proteins, Plasmacytoma, Protein Biosynthesis, RNA, Messenger, RNA, Neoplasm, RNA, Viral, Vesicular stomatitis Indiana virus, Viral Proteins
Abstract

Infection of MPC-11 mouse plasmacytoma cells by vesicular stomatitis virus results in 30 to 35% reduction in [35S]methionine incorporation into total proteins within 30 min postinfection. By 6 h postinfection, total protein synthesis is reduced by 80 to 90%. However, even by 30 min postinfection, a differential suppression of the synthesis of individual host protein is observed. The synthesis of the immunoglobin G (IgG) heavy chain (H), and, in particular, the synthesis of IgG light chain (L), is considerably more resistant to vesicular stomatitis virus-induced inhibition than is the synthesis of the non-IgG proteins as a whole; e.g., when the synthesis of non-IgG proteins was reduced by 41%, the synthesis of the H and L chains was reduced by 28 and 7%, respectively. Furthermore, these alterations in the relative synthesis of the L chain, H chain, and non-IgG are comparable to the alterations previously observed in uninfected MPC-11 cells when the overall rate of polypeptide chain initiation was selectively reduced (D.L. Nuss and G. Koch, 1976). These results are discussed in terms of the strategy of virus-directed suppression of host mRNA translation.

Alternate JournalJ. Virol.
PubMed ID183015
PubMed Central IDPMC354893