Correlation between three-dimensional structure and chemical reactivity of transfer RNA.

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TitleCorrelation between three-dimensional structure and chemical reactivity of transfer RNA.
Publication TypeJournal Article
Year of Publication1974
AuthorsRobertus, JD, Ladner, JE, Finch, JT, Rhodes, D, Brown, RS, Clark, BF, Klug, A
JournalNucleic Acids Res
Volume1
Issue7
Pagination927-32
Date Published1974 Jul
ISSN0305-1048
KeywordsBase Sequence, Binding Sites, Carbodiimides, Hydroxylamines, Models, Molecular, Molecular Sequence Data, Nucleic Acid Conformation, RNA, Fungal, RNA, Transfer, Phe, Saccharomyces cerevisiae
Abstract

The bases of yeast tRNA(Phe) which react with carbodiimide and methoxyamine have been determined and this information has been combined with chemical modification studies of other workers to produce a composite picture of base accessibility in this tRNA. The results are compared with the three-dimensional structure which we have recently determined. The bases which react chemically lie in exposed positions in the three-dimensional model and those which do not are either in the double helical stem regions or else are involved in maintaining the tertiary structure through pairing or stacking interactions.

Alternate JournalNucleic Acids Res.
PubMed ID10793725
PubMed Central IDPMC343396