Effects of dilute HCl on yeast tRNAPhe and E. coli tRNA1fMet.

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TitleEffects of dilute HCl on yeast tRNAPhe and E. coli tRNA1fMet.
Publication TypeJournal Article
Year of Publication1974
AuthorsLadner, JE, Schweizer, MP
JournalNucleic Acids Res
Date Published1974 Feb
KeywordsComputers, Deuterium, Dimethyl Sulfoxide, Electrophoresis, Polyacrylamide Gel, Escherichia coli, Formates, Hydrochloric Acid, Hydrogen-Ion Concentration, Magnetic Resonance Spectroscopy, Methionine, Nucleic Acid Conformation, Optical Rotation, Phenylalanine, RNA, Bacterial, RNA, Transfer, Saccharomyces cerevisiae, Solubility, Species Specificity, Spectrophotometry, Ultraviolet, Time Factors

HCl treatment of yeast tRNA(Phe) under conditions generally used for excision of ;Y' base results in structure and conformation changes as monitored by line widths in the PMR spectra at 220 MHz and by optical rotation. Like exposure of E. coli tRNA(fMet) (1) causes similar changes in the PMR spectra and optical rotation although no residues are eliminated. Electrophoresis in polyacrylamide gels provides evidence for aggregation in HCl-treated tRNA(fMet) (1). One must thus consider a general effect of HCl exposure as well as possible residue removal in assessing induced structural and conformation changes in tRNA.

Alternate JournalNucleic Acids Res.
PubMed ID4606505
PubMed Central IDPMC343337