Microneedles with intrinsic immunoadjuvant properties: microfabrication, protein stability, and modulated release.

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TitleMicroneedles with intrinsic immunoadjuvant properties: microfabrication, protein stability, and modulated release.
Publication TypeJournal Article
Year of Publication2011
AuthorsAndrianov, AK, Marin, A, Decollibus, DP
JournalPharm Res
Volume28
Issue1
Pagination58-65
Date Published2011 Jan
ISSN1573-904X
KeywordsAdjuvants, Immunologic, Chemistry, Pharmaceutical, Immunization, Injections, Intradermal, Microinjections, Needles, Organophosphorus Compounds, Polymers, Protein Stability, Serum Albumin, Bovine, Solubility, Technology, Pharmaceutical, Time Factors, Vaccines
Abstract

PURPOSE: Intradermal immunization using microneedles requires compatible immunoadjuvant system. To address this challenge, we investigated microneedles coated with polyphosphazene polyelectrolyte, which served both as microfabrication material and an immunoadjuvant compound.

METHODS: Coated microneedles were fabricated by depositing formulations containing Poly[di(carboxylatophenoxy)phosphazene], PCPP, on metal shafts, and their physico-chemical characterization was conducted.

RESULTS: Microfabrication of PCPP-coated microneedles exhibited strong dependence on protein-PCPP interactions in solutions and allowed for high efficiency of protein encapsulation. 70°C thermal inactivation studies demonstrated a remarkable increase in functional stability of protein in coated microneedles compared to solution formulation. A potential for modulation of protein release from coated microneedles has been demonstrated through ionic complexation of PCPP with small ions.

CONCLUSIONS: Microneedles containing PCPP coatings provide improved protein stability, modulated release, and protein-friendly microfabrication process.

DOI10.1007/s11095-010-0133-7
Alternate JournalPharm. Res.
PubMed ID20372988