|Title||Microneedles with intrinsic immunoadjuvant properties: microfabrication, protein stability, and modulated release.|
|Publication Type||Journal Article|
|Year of Publication||2011|
|Authors||Andrianov, AK, Marin, A, Decollibus, DP|
|Date Published||2011 Jan|
|Keywords||Adjuvants, Immunologic, Chemistry, Pharmaceutical, Immunization, Injections, Intradermal, Microinjections, Needles, Organophosphorus Compounds, Polymers, Protein Stability, Serum Albumin, Bovine, Solubility, Technology, Pharmaceutical, Time Factors, Vaccines|
PURPOSE: Intradermal immunization using microneedles requires compatible immunoadjuvant system. To address this challenge, we investigated microneedles coated with polyphosphazene polyelectrolyte, which served both as microfabrication material and an immunoadjuvant compound.
METHODS: Coated microneedles were fabricated by depositing formulations containing Poly[di(carboxylatophenoxy)phosphazene], PCPP, on metal shafts, and their physico-chemical characterization was conducted.
RESULTS: Microfabrication of PCPP-coated microneedles exhibited strong dependence on protein-PCPP interactions in solutions and allowed for high efficiency of protein encapsulation. 70°C thermal inactivation studies demonstrated a remarkable increase in functional stability of protein in coated microneedles compared to solution formulation. A potential for modulation of protein release from coated microneedles has been demonstrated through ionic complexation of PCPP with small ions.
CONCLUSIONS: Microneedles containing PCPP coatings provide improved protein stability, modulated release, and protein-friendly microfabrication process.
|Alternate Journal||Pharm. Res.|