Delineating antibody recognition in polyclonal sera from patterns of HIV-1 isolate neutralization.

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TitleDelineating antibody recognition in polyclonal sera from patterns of HIV-1 isolate neutralization.
Publication TypeJournal Article
Year of Publication2013
AuthorsGeorgiev, IS, Doria-Rose, NA, Zhou, T, Kwon, YDo, Staupe, RP, Moquin, S, Chuang, G-Y, Louder, MK, Schmidt, SD, Altae-Tran, HR, Bailer, RT, McKee, K, Nason, M, O'Dell, S, Ofek, GA, Pancera, M, Srivatsan, S, Shapiro, L, Connors, M, Migueles, SA, Morris, L, Nishimura, Y, Martin, MA, Mascola, JR, Kwong, PD
JournalScience
Volume340
Issue6133
Pagination751-6
Date Published2013 May 10
ISSN1095-9203
KeywordsAnimals, Antibodies, Neutralizing, Epitope Mapping, HIV Antibodies, HIV Infections, HIV-1, Humans, Immunodominant Epitopes, Macaca, Neutralization Tests, Protein Conformation, Serum
Abstract

Serum characterization and antibody isolation are transforming our understanding of the humoral immune response to viral infection. Here, we show that epitope specificities of HIV-1-neutralizing antibodies in serum can be elucidated from the serum pattern of neutralization against a diverse panel of HIV-1 isolates. We determined "neutralization fingerprints" for 30 neutralizing antibodies on a panel of 34 diverse HIV-1 strains and showed that similarity in neutralization fingerprint correlated with similarity in epitope. We used these fingerprints to delineate specificities of polyclonal sera from 24 HIV-1-infected donors and a chimeric siman-human immunodeficiency virus-infected macaque. Delineated specificities matched published specificities and were further confirmed by antibody isolation for two sera. Patterns of virus-isolate neutralization can thus afford a detailed epitope-specific understanding of neutralizing-antibody responses to viral infection.

DOI10.1126/science.1233989
Alternate JournalScience
PubMed ID23661761
Grant ListU19 AI51794 / AI / NIAID NIH HHS / United States
/ / Intramural NIH HHS / United States