Pollen viability of Polygala paniculata L. (Polygalaceae) using different staining methods.

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TitlePollen viability of Polygala paniculata L. (Polygalaceae) using different staining methods.
Publication TypeJournal Article
Year of Publication2012
AuthorsFrescura, VDal-Souto, Laughinghouse, HDail, Canto-Dorow, TScotti Do, Tedesco, SBosio
JournalBiocell
Volume36
Issue3
Pagination143-5
Date Published2012 Dec
ISSN0327-9545
KeywordsColoring Agents, Microscopy, Fluorescence, Plants, Medicinal, Pollen, Polygalaceae, Staining and Labeling
Abstract

Polygala paniculata L. is a medicinal plant that grows in the Brazilian Atlantic coast, known as 'barba-de-São-João', 'barba-de-bode', 'vassourinha branca', and 'mimosa'. In this study, pollen viability was estimated by three different staining methods: 2% acetic orcein, 2% acetic carmine, and Alexander's stain. The young inflorescences of twenty accessions were collected and fixed in a solution of ethanol: acetic acid (3:1) for 24 hours, then stored in ethanol 70% under refrigeration. Six slides per plant, two for each stain, were prepared by squashing, and 300 pollen grains per slide were analyzed. Pollen viability was high (> 70%) for most accessions of P. paniculata using the Alexander's stain, which proved the most adequate method to estimate pollen viability.

Alternate JournalBiocell
PubMed ID23682430