Crystal structure of a plectonemic RNA supercoil.

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TitleCrystal structure of a plectonemic RNA supercoil.
Publication TypeJournal Article
Year of Publication2012
AuthorsStagno, JR, Ma, B, Li, J, Altieri, AS, R Byrd, A, Ji, X
JournalNat Commun
Volume3
Pagination901
Date Published2012
ISSN2041-1723
KeywordsCrystallography, X-Ray, Mass Spectrometry, Molecular Dynamics Simulation, Nucleic Acid Conformation, RNA, RNA-Binding Proteins, Structure-Activity Relationship
Abstract

Genome packaging is an essential housekeeping process in virtually all organisms for proper storage and maintenance of genetic information. Although the extent and mechanisms of packaging vary, the process involves the formation of nucleic-acid superstructures. Crystal structures of DNA coiled coils indicate that their geometries can vary according to sequence and/or the presence of stabilizers such as proteins or small molecules. However, such superstructures have not been revealed for RNA. Here we report the crystal structure of an RNA supercoil, which displays one level higher molecular organization than previously reported structures of DNA coiled coils. In the presence of an RNA-binding protein, two interlocking RNA coiled coils of double-stranded RNA, a 'coil of coiled coils', form a plectonemic supercoil. Molecular dynamics simulations suggest that protein-RNA interaction is required for the stability of the supercoiled RNA. This study provides structural insight into higher order packaging mechanisms of nucleic acids.

DOI10.1038/ncomms1903
Alternate JournalNat Commun
PubMed ID22692544
PubMed Central IDPMC3518024
Grant ListZIA BC010326-12 / / Intramural NIH HHS / United States
ZIA BC010326-13 / / Intramural NIH HHS / United States