A piRNA-like small RNA interacts with and modulates p-ERM proteins in human somatic cells.

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TitleA piRNA-like small RNA interacts with and modulates p-ERM proteins in human somatic cells.
Publication TypeJournal Article
Year of Publication2015
AuthorsMei, Y, Wang, Y, Kumari, P, Shetty, ACarl, Clark, D, Gable, T, Mackerell, AD, Ma, MZ, Weber, DJ, Yang, AJ, Edelman, MJ, Mao, L
JournalNat Commun
Volume6
Pagination7316
Date Published2015 Jun 22
ISSN2041-1723
KeywordsActins, Blotting, Northern, Bronchi, Carcinoma, Non-Small-Cell Lung, Cell Line, Cell Line, Tumor, Cytoskeletal Proteins, Gene Expression Profiling, Humans, Immunoprecipitation, Lung Neoplasms, Mass Spectrometry, Membrane Proteins, Microfilament Proteins, Phosphoproteins, Respiratory Mucosa, Reverse Transcriptase Polymerase Chain Reaction, RNA, Small Interfering, Sodium-Hydrogen Antiporter
Abstract

PIWI-interacting RNAs (piRNAs) are thought to silence transposon and gene expression during development. However, the roles of piRNAs in somatic tissues are largely unknown. Here we report the identification of 555 piRNAs in human lung bronchial epithelial (HBE) and non-small cell lung cancer (NSCLC) cell lines, including 295 that do not exist in databases termed as piRNA-like sncRNAs or piRNA-Ls. Distinctive piRNA/piRNA-L expression patterns are observed between HBE and NSCLC cells. piRNA-like-163 (piR-L-163), the top downregulated piRNA-L in NSCLC cells, binds directly to phosphorylated ERM proteins (p-ERM), which is dependent on the central part of UUNNUUUNNUU motif in piR-L-163 and the RRRKPDT element in ERM. The piR-L-163/p-ERM interaction is critical for p-ERM's binding capability to filamentous actin (F-actin) and ERM-binding phosphoprotein 50 (EBP50). Thus, piRNA/piRNA-L may play a regulatory role through direct interaction with proteins in physiological and pathophysiological conditions.

DOI10.1038/ncomms8316
Alternate JournalNat Commun
PubMed ID26095918
PubMed Central IDPMC4557300
Grant ListR01 GM051501 / GM / NIGMS NIH HHS / United States