Purification, crystallization, and preliminary X-ray crystallographic analysis of the Group III chaperonin from Carboxydothermus hydrogenoformans.

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TitlePurification, crystallization, and preliminary X-ray crystallographic analysis of the Group III chaperonin from Carboxydothermus hydrogenoformans.
Publication TypeJournal Article
Year of Publication2016
AuthorsAn, YJun, Rowland, SE, Robb, FT, Cha, S-S
JournalJ Microbiol
Volume54
Issue6
Pagination440-4
Date Published2016 Jun
ISSN1976-3794
KeywordsAdenosine Diphosphate, Adenosine Triphosphate, Amino Acid Sequence, Bacterial Proteins, Chaperonins, Crystallography, X-Ray, Models, Molecular, Molecular Sequence Data, Sequence Alignment, Thermoanaerobacterium
Abstract

Chaperonins (CPNs) are megadalton sized ATP-dependent nanomachines that facilitate protein folding through complex cycles of complex allosteric articulation. They consist of two back-to-back stacked multisubunit rings. CPNs are usually classified into Group I and Group II. Here, we report the crystallization of both the AMPPNP (an ATP analogue) and ADP bound forms of a novel CPN, classified as belonging to a third Group, recently discovered in the extreme thermophile Carboxydothermus hydrogenoformans. Crystals of the two forms were grown by the vapor batch crystallization method at 295 K. Crystals of the Ch-CPN/AMPPNP complex diffracted to 3.0 Å resolution and belonged to the space group P422, with unit-cell parameters a = b = 186.166, c = 160.742 Å. Assuming the presence of four molecules in the asymmetric unit, the solvent content was estimated to be about 60.02%. Crystals of the Ch-CPN/ADP complex diffracted to 4.0 Å resolution and belonged to the space group P4212, with unit-cell parameters a = b = 209.780, c = 169.813Å. Assuming the presence of four molecules in the asymmetric unit, the solvent content was estimated to be about 70.19%.

DOI10.1007/s12275-016-6089-5
Alternate JournalJ. Microbiol.
PubMed ID27225461