Nucleotide sequence and analysis of the Vibrio alginolyticus sucrase gene (scrB).

Printer-friendly versionPrinter-friendly versionPDF versionPDF version
TitleNucleotide sequence and analysis of the Vibrio alginolyticus sucrase gene (scrB).
Publication TypeJournal Article
Year of Publication1989
AuthorsScholle, RR, Robb, SM, Robb, FT, Woods, DR
JournalGene
Volume80
Issue1
Pagination49-56
Date Published1989 Aug 01
ISSN0378-1119
KeywordsAmino Acid Sequence, Bacillus, Base Sequence, Cloning, Molecular, DNA Transposable Elements, DNA, Bacterial, Gene Expression Regulation, Genes, Bacterial, Molecular Sequence Data, Mutation, Plasmids, Restriction Mapping, Saccharomyces cerevisiae, Sequence Homology, Nucleic Acid, Sucrase, Sucrose, Vibrio
Abstract

The nucleotide sequence of a 2.119-kb DNA fragment containing the Vibrio alginolyticus sucrase gene (scrB) was determined. The complete sequence (484 aa residues) of the sucrase was deduced and homology was detected between the sucrase enzymes from V. alginolyticus and the Gram-positive bacteria Bacillus subtilis and Streptococcus mutans. In Escherichia coli cells the cloned V. alginolyticus sucrase is translocated to the periplasm. Transposon phoA mutagenesis experiments strongly suggested that V. alginolyticus sucrase in E. coli is not exported across the cytoplasmic membrane by means of a typical signal sequence.

Alternate JournalGene
PubMed ID2551785