Phage growth characteristics on stationary phase Achromobacter cells.

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TitlePhage growth characteristics on stationary phase Achromobacter cells.
Publication TypeJournal Article
Year of Publication1978
AuthorsRobb, SM, Woods, DR, Robb, FT
JournalJ Gen Virol
Volume41
Issue2
Pagination265-72
Date Published1978 Nov
ISSN0022-1317
KeywordsAdsorption, Alcaligenes, Bacteriophages, Virus Replication
Abstract

The growth characteristics of alpha3a bacteriophage on stationary phase Achromobacter strain 14 are described. Phage alpha3a growth on stationary phase cells is characterized by a long and variable latent period of 6 to 9 h and an increased burst size of 710 p.f.u./cell as compared with 153 p.f.u./cell in exponential wild type cells. During the latent period the infected cells are very sensitive to changes in growth conditions and in particular, dilution. Pre-conditioning of the bacterial cells by allowing them to stand for 24 h after shaking for 3 days is an important aspect of the stationary phase phage growth system. Cells which have been allowed to stand retain the ability to be infected and to support phage growth for at least 16 days. Shaking cultures gradually lose the ability to support phage growth but the phage can persist in the host cell for 10 days until removal from shaking when the lytic cycle can proceed after allowing the cultures to stand.

DOI10.1099/0022-1317-41-2-265
Alternate JournalJ. Gen. Virol.
PubMed ID722281