Drug delivery in pluronic micelles: effect of high-frequency ultrasound on drug release from micelles and intracellular uptake.

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TitleDrug delivery in pluronic micelles: effect of high-frequency ultrasound on drug release from micelles and intracellular uptake.
Publication TypeJournal Article
Year of Publication2002
AuthorsMarin, A, Sun, H, Husseini, GA, Pitt, WG, Christensen, DA, Rapoport, NY
JournalJ Control Release
Volume84
Issue1-2
Pagination39-47
Date Published2002 Nov 07
ISSN0168-3659
KeywordsAlgorithms, Antibiotics, Antineoplastic, Breast Neoplasms, Doxorubicin, Drug Delivery Systems, Electron Spin Resonance Spectroscopy, Excipients, Female, Flow Cytometry, HL-60 Cells, Humans, Leukemia, Promyelocytic, Acute, Micelles, Ovarian Neoplasms, Poloxamer, Spectrometry, Fluorescence, Surface Properties, Ultrasonics
Abstract

The effect of high-frequency ultrasound on doxorubicin (DOX) release from Pluronic micelles and intracellular DOX uptake was studied for promyelocytic leukemia HL-60 cells, ovarian carcinoma drug-sensitive and multidrug-resistant (MDR) cells (A2780 and A2780/ADR, respectively), and breast cancer MCF-7 cells. Cavitation events initiated by high-frequency ultrasound were recorded by radical trapping. The onset of transient cavitation and DOX release from micelles were observed at much higher power densities than at low-frequency ultrasound (20-100 kHz). Even a short (15-30 s) exposure to high-frequency ultrasound significantly enhanced the intracellular DOX uptake from PBS, RPMI 1640, and Pluronic micelles. The mechanisms of the observed effects are discussed.

Alternate JournalJ Control Release
PubMed ID12399166
Grant ListCA 76562 / CA / NCI NIH HHS / United States