Nonuniform Sampling in Multidimensional NMR for Improving Spectral Sensitivity.

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TitleNonuniform Sampling in Multidimensional NMR for Improving Spectral Sensitivity.
Publication TypeJournal Article
Year of Publication2018
AuthorsZambrello, MA, Schuyler, AD, Maciejewski, MW, Delaglio, F, Bezsonova, I, Hoch, JC
Date Published2018 Mar 06

The development of multidimensional NMR spectroscopy enabled an explosion of structural and dynamical investigations on proteins and other biomacromolecules. Practical limitations on data sampling, based on the Jeener paradigm of parametric sampling of indirect time domains, have long placed limits on resolution in the corresponding frequency dimensions. The emergence of nonuniform sampling (NUS) in indirect time dimensions circumvents those limitations, affording high resolution spectra from short data records collected in practically realized measurement times. In addition to substantially improved resolution, NUS can also be exploited to improve sensitivity, with gains comparable to those obtained using cryogenically cooled probes. We describe a general approach for acquiring and processing multidimensional NUS NMR data for improving sensitivity.

Alternate JournalMethods
PubMed ID29522805