Biofabricating Functional Soft Matter Using Protein Engineering to Enable Enzymatic Assembly.

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TitleBiofabricating Functional Soft Matter Using Protein Engineering to Enable Enzymatic Assembly.
Publication TypeJournal Article
Year of Publication2018
AuthorsLiu, Y, Wu, H-C, Bhokisham, N, Li, J, Hong, K-L, Quan, DN, Tsao, C-Y, Bentley, WE, Payne, GF
JournalBioconjug Chem
Date Published2018 May 16
ISSN1520-4812
Abstract

Biology often provides the inspiration for functional soft matter, but biology can do more: it can provide the raw materials and mechanisms for hierarchical assembly. Biology uses polymers to perform various functions, and biologically derived polymers can serve as sustainable, self-assembling, and high-performance materials platforms for life-science applications. Biology employs enzymes for site-specific reactions that are used to both disassemble and assemble biopolymers both to and from component parts. By exploiting protein engineering methodologies, proteins can be modified to make them more susceptible to biology's native enzymatic activities. They can be engineered with fusion tags that provide (short sequences of amino acids at the C- and/or N- termini) that provide the accessible residues for the assembling enzymes to recognize and react with. This "biobased" fabrication not only allows biology's nanoscale components (i.e., proteins) to be engineered, but also provides the means to organize these components into the hierarchical structures that are prevalent in life.

DOI10.1021/acs.bioconjchem.8b00197
Alternate JournalBioconjug. Chem.
PubMed ID29745651