Fabrication of Tobacco Mosaic Virus-Like Nanorods for Peptide Display.

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TitleFabrication of Tobacco Mosaic Virus-Like Nanorods for Peptide Display.
Publication TypeJournal Article
Year of Publication2018
AuthorsLarkin, EJ, Brown, AD, Culver, JN
JournalMethods Mol Biol
Volume1776
Pagination51-60
Date Published2018
ISSN1940-6029
Abstract

Virus-like particles (VLPs) are genome-free protein shells assembled from virus coat proteins (CPs). The uniform and nanoscale structure of VLPs combined with their noninfectious nature have made them ideal candidates for the display of functional peptides. While the vast majority of VLPs are derived from spherical viruses, tobacco mosaic virus (TMV) produces a rod-shaped particle with a hollow central channel. However, under physiological conditions the TMV CP forms only disk-shaped macromolecules. Here, we describe the design, construction, purification, and processing of rod-shaped TMV-VLPs using a simple bacterial expression system. The robust nature of this system allows for the display of functional peptides and molecules on the outer surface of this novel VLP.

DOI10.1007/978-1-4939-7808-3_4
Alternate JournalMethods Mol. Biol.
PubMed ID29869234