Electrodeposition of a magnetic and redox-active chitosan film for capturing and sensing metabolic active bacteria.

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TitleElectrodeposition of a magnetic and redox-active chitosan film for capturing and sensing metabolic active bacteria.
Publication TypeJournal Article
Year of Publication2018
AuthorsLi, Y, Liu, Y, Kim, E, Song, Y, Tsao, C-Y, Teng, Z, Gao, T, Mei, L, Bentley, WE, Payne, GF, Wang, Q
JournalCarbohydr Polym
Volume195
Pagination505-514
Date Published2018 Sep 01
ISSN1879-1344
Abstract

Rapid and portable detection of viable pathogen is highly desired to minimize the risk of foodborne pathogen outbreaks. Here we report a proof-of-concept fabrication methodology of a multifunctional film that allows established methods from bacterial recognition (antibodies) and nanotechnology (magnetic nanoparticles) to be coupled with electrochemical signal processing methods for detection of viable bacteria. Specifically, we enlist a sequence of externally applied electrical and magnetic signals to: i) guide the self-assembly of stimuli-responsive biopolymer; ii) incorporate magnetic nanoparticles to form a magnetic layer; iii) electro-synthesize a signal processing layer (redox-capacitor). The function of the magnetic layer is collecting and concentrating MMP-bacteria through magnetic attractions between MMP-bacteria and the magnetic layer. The function of the signal processing layer is amplifying electrochemical detection of the collected bacteria by engaging the redox-active mediators with the redox capacitor. Importantly, the fabrication demonstrated here is simple, controllable, and reagentless.

DOI10.1016/j.carbpol.2018.04.096
Alternate JournalCarbohydr Polym
PubMed ID29805005