Structure of the Fc fragment of the NIST reference antibody RM8671.

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TitleStructure of the Fc fragment of the NIST reference antibody RM8671.
Publication TypeJournal Article
Year of Publication2018
AuthorsD Gallagher, T, Galvin, CV, Karageorgos, I
JournalActa Crystallogr F Struct Biol Commun
Volume74
IssuePt 9
Pagination524-529
Date Published2018 Sep 01
ISSN2053-230X
Abstract

As the link between antigen binding and immune activation, the antibody Fc region has received extensive structural study. In this report, the structure of the Fc fragment of the NIST IgG1 mAb (reference material 8671) is described at 2.1 Å resolution in space group P222, with approximate unit-cell parameters a = 50, b = 80, c = 138 Å. Prior Fc structures with a wide variety of modifications are also surveyed, focusing on those in the same crystal form. To facilitate the analysis of conformations, a reference frame and a two-parameter metric are proposed, considering the CH2 domains as mobile with respect to a fixed dimeric CH3 core. Over several human Fc structures, a significant variation in Fc elbow conformations is observed, which may serve to facilitate the regulation of Fc effector signaling.

DOI10.1107/S2053230X18009834
Alternate JournalActa Crystallogr F Struct Biol Commun
PubMed ID30198883
Grant ListDE-AC02-06CH11357 / / U.S. Department of Energy, Office of Biological and Environmental Research /