Strategies for Development of a Next-Generation Protein Sequencing Platform.

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TitleStrategies for Development of a Next-Generation Protein Sequencing Platform.
Publication TypeJournal Article
Year of Publication2019
AuthorsCallahan, N, Tullman, J, Kelman, Z, Marino, JP
JournalTrends Biochem Sci
Date Published2019 Oct 29
ISSN0968-0004
Abstract

Proteomic analysis can be a critical bottleneck in cellular characterization. The current paradigm relies primarily on mass spectrometry of peptides and affinity reagents (i.e., antibodies), both of which require a priori knowledge of the sample. An unbiased protein sequencing method, with a dynamic range that covers the full range of protein concentrations in proteomes, would revolutionize the field of proteomics, allowing a more facile characterization of novel gene products and subcellular complexes. To this end, several new platforms based on single-molecule protein-sequencing approaches have been proposed. This review summarizes four of these approaches, highlighting advantages, limitations, and challenges for each method towards advancing as a core technology for next-generation protein sequencing.

DOI10.1016/j.tibs.2019.09.005
Alternate JournalTrends Biochem. Sci.
PubMed ID31676211