Zvi Kelman

Research Biologist

Kelman Group


Email: zkelman@umd.edu

Call: (240) 314-6294


  • Ph.D., Molecular Biology, Cornell University Graduate School of Medical Sciences, New York
  • MSc., Cell Biology, Weizmann Institute of Science, Rehovot, Israel
  • BSc., Agriculture, Hebrew University of Jerusalem, Rehovot, Israel


Dr. Zvi Kelman’s research is focused on developing tools and reagents for the labeling of biomolecules with stable isotopes to support biophysical and bioanalytical measurements. The NIST Biomolecular Labeling Laboratory (BL2) also provides support to external users through a proposal process. The laboratory has the equipment and reagents necessary for production, purification, and characterization of labeled proteins and other biomolecules, including peptides and nucleic acids. A part of these efforts includes the labeling of monoclonal antibodies (mAbs).  mAbs are commonly developed by the pharmaceutical industry as drugs to fight a large variety of diseases, including cancer and autoimmune disease. Some structural studies on mAbs will benefit from the ability to label the antibodies with stable isotopes. The lab is developing tools and reagents for bacteria, yeast, plant and mammalian cell expression and purification of labeled mAbs.

Another project pursued by the lab is the development of tools and reagents for next-generation protein sequencing.  Next-gen protein sequencing is an emerging technology that shows promise to revolutionize the field of proteomics, as next-generation DNA and RNA sequencing did for the fields of genomics and transcriptomics.

The emerging landscape of single-molecule protein sequencing technologies.
Recombinant expression of computationally designed peptide-bundlemers in Escherichia coli.
Creation and filtering of a recurrent spectral library of CHO cell metabolites and media components.
Leveraging nature's biomolecular designs in next-generation protein sequencing reagent development.
Unwinding 20 Years of the Archaeal Minichromosome Maintenance Helicase.
Strategies for Development of a Next-Generation Protein Sequencing Platform.
Characterization of the internal translation initiation region in monoclonal antibodies expressed in Escherichia coli.
Editorial: The DNA Replication Machinery as Therapeutic Targets.
Engineering ClpS for selective and enhanced N-terminal amino acid binding.
Neutron scattering in the biological sciences: progress and prospects.
DNA Sliding Clamps as Therapeutic Targets.
Platform development for expression and purification of stable isotope labeled monoclonal antibodies in Escherichia coli.
Heterologous recombinant expression of non-originator NISTmAb.
Archaeal DNA replication and repair: new genetic, biophysical and molecular tools for discovering and characterizing enzymes, pathways and mechanisms.
Do Archaea Need an Origin of Replication?
Genome Replication in Thermococcus kodakarensis Independent of Cdc6 and an Origin of Replication.
The GAN Exonuclease or the Flap Endonuclease Fen1 and RNase HII Are Necessary for Viability of Thermococcus kodakarensis.
A small protein inhibits proliferating cell nuclear antigen by breaking the DNA clamp.