IBBR Seminar Series

NIST BMD/IBBR Seminar: Investigating lipoproteins using electrospray-differential mobility analysis and quantitative LC-MS/MS

Multiple lipoproteins are known to be causal risk factors for cardiovascular disease (CVD). While LDL cholesterol (LDL-C) has been causally linked to CVD risk, the link of HDL-C, the “good cholesterol”, to CVD is much more complicated and the HDL-C as a metric of HDL’s antiatherogenic properties has been put in question both by genetics studies and by failure of pharmacologic elevation of HDL-C to lower CVD risk. We’ve developed a novel measurement of HDL that directly measures concentration of HDL particles (including individual subspecies) as opposed to the HDL cholesterol content.

NIST BMD/IBBR Seminar: "HDL: Lipid Nanoparticles at the Intersection of Health and Disease"

Although much is known about lipoprotein biology, particularly low-density lipoproteins (LDL), because of their strong associations with cardiovascular disease, many questions about the less well-understood high density lipoproteins (HDL) remain unanswered. Lipoproteins are lipid nanoparticles that range in size from the smallest HDL particles which are 5nm in diameter, to the largest intestinally-derived chylomicron particles which can be as large as 1000nm in diameter.

NIST BMD/IBBR Seminar: “New Technologies to Study Archaeal DNA Replication and Repair”

DNA replication and repair are essential biological processes needed for the survival of all organisms across all domains of life. Interestingly, a variety of archaeal species thrive in extreme environments, including high temperature, high salt, and low pH, and must maintain their genome under such extremes.  In this talk I will present two technologies developed and utilized by our lab at New England Biolabs to study DNA replication and repair in hyperthermophilic Archaea.