PEPM/ICL Superfamily

The PEP mutase/isocitrate lyase superfamily includes enzyme families that share amino acid sequence homology. They also adopt the same three-dimensional scaffold and exhibit common features in their active sites. Members of the superfamily catalyze a wide range of biochemical reactions involving P-C and C-C bond cleavage/formation. Moreover, the functions of some of the families within the superfamily are unknown. With the advent of genome sequencing, the superfamily contains hundreds of sequences, both from prokaryotes and eukaryotes. These sequences are sometimes wrongly annotated in sequence databases.
The goals of our studies are to obtain a representative structure of each function family, to elucidate the catalytic mechanism of each reaction, and develop structure-based signature sequences that will enable to correctly annotate the function of each superfamily member.