The emerging landscape of single-molecule protein sequencing technologies.

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TitleThe emerging landscape of single-molecule protein sequencing technologies.
Publication TypeJournal Article
Year of Publication2021
AuthorsAlfaro, JAntonio, Bohländer, P, Dai, M, Filius, M, Howard, CJ, van Kooten, XF, Ohayon, S, Pomorski, A, Schmid, S, Aksimentiev, A, Anslyn, EV, Bedran, G, Cao, C, Chinappi, M, Coyaud, E, Dekker, C, Dittmar, G, Drachman, N, Eelkema, R, Goodlett, D, Hentz, S, Kalathiya, U, Kelleher, NL, Kelly, RT, Kelman, Z, Kim, SHyun, Kuster, B, Rodriguez-Larrea, D, Lindsay, S, Maglia, G, Marcotte, EM, Marino, JP, Masselon, C, Mayer, M, Samaras, P, Sarthak, K, Sepiashvili, L, Stein, D, Wanunu, M, Wilhelm, M, Yin, P, Meller, A, Joo, C
JournalNat Methods
Volume18
Issue6
Pagination604-617
Date Published2021 Jun
ISSN1548-7105
Abstract

Single-cell profiling methods have had a profound impact on the understanding of cellular heterogeneity. While genomes and transcriptomes can be explored at the single-cell level, single-cell profiling of proteomes is not yet established. Here we describe new single-molecule protein sequencing and identification technologies alongside innovations in mass spectrometry that will eventually enable broad sequence coverage in single-cell profiling. These technologies will in turn facilitate biological discovery and open new avenues for ultrasensitive disease diagnostics.

DOI10.1038/s41592-021-01143-1
Alternate JournalNat Methods
PubMed ID34099939
PubMed Central IDPMC8223677
Grant List17924 / / Michael J. Fox Foundation for Parkinson's Research (Michael J. Fox Foundation) /
883684 / / EC | EU Framework Programme for Research and Innovation H2020 | H2020 Priority Excellent Science | H2020 European Research Council (H2020 Excellent Science - European Research Council) /
C17/BM/11642138 / / Fonds National de la Recherche Luxembourg (National Research Fund) /
F-0046 / / Welch Foundation /
HG009186 / / U.S. Department of Health & Human Services | National Institutes of Health (NIH) /
MAB/2017/03 / / Fundacja na rzecz Nauki Polskiej (Foundation for Polish Science) /
UH3 CA246635 / CA / NCI NIH HHS / United States
PHY-1430124 / / National Science Foundation (NSF) /
R01 GM138931 / GM / NIGMS NIH HHS / United States
No. 833399-ERC / / EC | Horizon 2020 Framework Programme (EU Framework Programme for Research and Innovation H2020) /
11715 / / Paul G. Allen Family Foundation /
RGP0026/2019 / / Human Frontier Science Program (HFSP) /
F1515 / / Welch Foundation /
P41 GM108569 / GM / NIGMS NIH HHS / United States
R01 DK110520 / DK / NIDDK NIH HHS / United States
GA 616251 / / EC | EU Framework Programme for Research and Innovation H2020 | H2020 Priority Excellent Science | H2020 European Research Council (H2020 Excellent Science - European Research Council) /
W911NF-12-1-0390 / / United States Department of Defense | United States Army | U.S. Army Research, Development and Engineering Command | Army Research Office (ARO) /
R35 GM122480 / GM / NIGMS NIH HHS / United States
3485/19 / / Israel Science Foundation (ISF) /
grant no. 200021-169304 / / Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (Swiss National Science Foundation) /