A new approach to quantification of mAb aggregates using peptide affinity probes

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TitleA new approach to quantification of mAb aggregates using peptide affinity probes
Publication TypeJournal Article
Year of Publication2017
AuthorsCheung, CSF, Anderson, KW, Patel, PM, Cade, KL, Phinney, KW, Turko, IV
JournalSci Rep
Volume7
Pagination42497
Date Published2017 Feb 10
ISSN2045-2322
Abstract

Using mAbs as therapeutic molecules is complicated by the propensity of mAbs to aggregate at elevated concentrations, which can lead to a variety of adverse events in treatment. Here, we describe a proof-of-concept for new methodology to detect and quantify mAb aggregation. Assay development included using an aggregated mAb as bait for screening of phage display peptide library and identifying those peptides with random sequence which can recognize mAb aggregates. Once identified, the selected peptides can be used for developing quantitative methods to assess mAb aggregation. Results indicate that a peptide binding method coupled with mass spectrometric detection of bound peptide can quantify mAb aggregation and potentially be useful for monitoring aggregation propensity of therapeutic protein candidates.

DOI10.1038/srep42497
Alternate JournalSci Rep
Refereed DesignationRefereed
PubMed ID28186164