Regulation of ribosomal RNA transcription by growth rate of the hyperthermophilic Archaeon, Pyrococcus furiosus.

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TitleRegulation of ribosomal RNA transcription by growth rate of the hyperthermophilic Archaeon, Pyrococcus furiosus.
Publication TypeJournal Article
Year of Publication1993
AuthorsDiRuggiero, J, Achenbach, LA, Brown, SH, Kelly, RM, Robb, FT
JournalFEMS Microbiol Lett
Volume111
Issue2-3
Pagination159-64
Date Published1993 Aug 01
ISSN0378-1097
KeywordsArchaea, Base Sequence, Genes, Bacterial, Hot Temperature, Molecular Sequence Data, Oligodeoxyribonucleotides, RNA, Bacterial, RNA, Ribosomal, RNA, Ribosomal, 16S, RNA, Ribosomal, 23S, Transcription, Genetic
Abstract

We have studied the single rRNA gene cluster from the Archaeon, Pyrococcus furiosus. This isolate grows optimally at 100 degrees C and is thus a hyperthermophile. In P. furiosus, transcription of 16S rRNA is subject to regulation over a 7.5-fold range in response to a 20-fold increase in growth rate. The single cluster encoding the 16S and 23S rRNA genes of P. furiosus was cloned and the 1.9 kb region upstream of the 16S rRNA gene was sequenced.

Alternate JournalFEMS Microbiol. Lett.
PubMed ID7691680