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Zvi Kelman
Research Biologist
Kelman Group (240) 314-6294 zkelman@umd.eduDr. Zvi Kelman’s research is focused on developing tools and reagents for the labeling of biomolecules with stable isotopes to support biophysical and bioanalytical measurements. The NIST Biomolecular Labeling Laboratory (BL2) also provides support to external users through a proposal process. The laboratory has the equipment and reagents necessary for production, purification, and characterization of labeled proteins and other biomolecules, including peptides and nucleic acids. A part of these efforts includes the labeling of monoclonal antibodies (mAbs). mAbs are commonly developed by the pharmaceutical industry as drugs to fight a large variety of diseases, including cancer and autoimmune disease. Some structural studies on mAbs will benefit from the ability to label the antibodies with stable isotopes. The lab is developing tools and reagents for bacteria, yeast, plant and mammalian cell expression and purification of labeled mAbs.
Another project pursued by the lab is the development of tools and reagents for next-generation protein sequencing. Next-gen protein sequencing is an emerging technology that shows promise to revolutionize the field of proteomics, as next-generation DNA and RNA sequencing did for the fields of genomics and transcriptomics.
Publications
- Strategies for Development of a Next-Generation Protein Sequencing Platform.
- Characterization of the internal translation initiation region in monoclonal antibodies expressed in Escherichia coli.
- Editorial: The DNA Replication Machinery as Therapeutic Targets.
- Engineering ClpS for selective and enhanced N-terminal amino acid binding.
- Neutron scattering in the biological sciences: progress and prospects.
- DNA Sliding Clamps as Therapeutic Targets.
- Platform development for expression and purification of stable isotope labeled monoclonal antibodies in Escherichia coli.
- Heterologous recombinant expression of non-originator NISTmAb.
- Archaeal DNA replication and repair: new genetic, biophysical and molecular tools for discovering and characterizing enzymes, pathways and mechanisms.
- A small protein inhibits proliferating cell nuclear antigen by breaking the DNA clamp.
- High-temperature single-molecule kinetic analysis of thermophilic archaeal MCM helicases.
- A small protein inhibits proliferating cell nuclear antigen by breaking the DNA clamp.
- Evaluation of microbial qPCR workflows using engineered Saccharomyces cerevisiae.
- Preface.
- DNA polymerases in biotechnology.
- Archaeal DNA replication.
- Detection of contaminating enzymatic activity in plant-derived recombinant biotechnology products.
- Rolf Bernander (1956-2014): pioneer of the archaeal cell cycle.
- The solution structure of full-length dodecameric MCM by SANS and molecular modeling.
- Characterization of family D DNA polymerase from Thermococcus sp. 9°N.
- A novel mechanism for regulating the activity of proliferating cell nuclear antigen by a small protein.
- Thermococcus kodakarensis has two functional PCNA homologs but only one is required for viability.
- Archaeal DNA polymerase D but not DNA polymerase B is required for genome replication in Thermococcus kodakarensis.
- Thermococcus kodakarensis DNA replication.
- The archaeal DNA replication machinery: past, present and future.
- The modern "3G" age of archaeal molecular biology.
- DNA repair and replication fork helicases are differentially affected by alkyl phosphotriester lesion.
- Formation of dAMP-glycerol and dAMP-Tris derivatives by Thermococcus kodakaraensis DNA primase.
- Characterization of DNA primase complex isolated from the archaeon, Thermococcus kodakaraensis.
- The CMG (CDC45/RecJ, MCM, GINS) complex is a conserved component of the DNA replication system in all archaea and eukaryotes.
- Atomistic ensemble modeling and small-angle neutron scattering of intrinsically disordered protein complexes: applied to minichromosome maintenance protein.
- A nuclear magnetic resonance based approach to accurate functional annotation of putative enzymes in the methanogen Methanosarcina acetivorans.
- Thermococcus kodakarensis encodes three MCM homologs but only one is essential.
- Structures of p63 DNA binding domain in complexes with half-site and with spacer-containing full response elements.
- A novel DNA nuclease is stimulated by association with the GINS complex.
- Mutational analysis of conserved aspartic acid residues in the Methanothermobacter thermautotrophicus MCM helicase.
- Crystal structures of two active proliferating cell nuclear antigens (PCNAs) encoded by Thermococcus kodakaraensis.
- The archaeal PCNA proteins.
- Different residues on the surface of the Methanothermobacter thermautotrophicus MCM helicase interact with single- and double-stranded DNA.
- Affinity purification of an archaeal DNA replication protein network.
- Helicase ATPase activity of the Tobacco mosaic virus 126-kDa protein modulates replicase complex assembly.
- Cell cycle regulated genes in the three domains of life.
- Low cobalamin levels associated with sickle cell disease: Contrasting origins and clinical meanings in two instructive patients.
- Alternative mechanisms for coordinating polymerase alpha and MCM helicase.
- Genomic mutations associated with mild and severe deficiencies of transcobalamin I (haptocorrin) that cause mildly and severely low serum cobalamin levels.
- A biochemically active MCM-like helicase in Bacillus cereus.
- Unwinding the structure and function of the archaeal MCM helicase.
- ATP hydrolysis and DNA binding confer thermostability on the MCM helicase.
- How is the archaeal MCM helicase assembled at the origin? Possible mechanisms.
- Cloning, Purification, and Partial Characterization of the Halobacterium sp. NRC-1 Minichromosome Maintenance (MCM) Helicase.
- The Methanothermobacter thermautotrophicus MCM helicase is active as a hexameric ring.
- Biochemical characterization of the minichromosome maintenance protein from the archaeon Thermoplasma acidophilum.
- Thermoplasma acidophilum Cdc6 protein stimulates MCM helicase activity by regulating its ATPase activity.
- Cryo-electron microscopy reveals a novel DNA-binding site on the MCM helicase.
- Studies with the human cohesin establishment factor, ChlR1. Association of ChlR1 with Ctf18-RFC and Fen1.
- The Methanothermobacter thermautotrophicus Cdc6-2 protein, the putative helicase loader, dissociates the minichromosome maintenance helicase.
- Coupling of DNA binding and helicase activity is mediated by a conserved loop in the MCM protein.
- Archaeal minichromosome maintenance (MCM) helicase can unwind DNA bound by archaeal histones and transcription factors.
- DNA unwinding assay using streptavidin-bound oligonucleotides.
- Stimulation of MCM helicase activity by a Cdc6 protein in the archaeon Thermoplasma acidophilum.
- The replicative helicases of bacteria, archaea, and eukarya can unwind RNA-DNA hybrid substrates.
- DNA binding by the Methanothermobacter thermautotrophicus Cdc6 protein is inhibited by the minichromosome maintenance helicase.
- Archaeal DNA replication and repair.
- Interactions between the archaeal Cdc6 and MCM proteins modulate their biochemical properties.
- Association between Archaeal prolyl- and leucyl-tRNA synthetases enhances tRNA(Pro) aminoacylation.
- Structural polymorphism of Methanothermobacter thermautotrophicus MCM.
- Use of a restriction enzyme-digested PCR product as substrate for helicase assays.
- Multiple origins of replication in archaea.
- Novel structure and nucleotide binding properties of HI1480 from Haemophilus influenzae: a protein with no known sequence homologues.
- Biochemical characterization of the Methanothermobacter thermautotrophicus minichromosome maintenance (MCM) helicase N-terminal domains.
- Crystal structure of the chi:psi sub-assembly of the Escherichia coli DNA polymerase clamp-loader complex.
- Archeal DNA replication: eukaryal proteins in a bacterial context.
- Substrate requirements for duplex DNA translocation by the eukaryal and archaeal minichromosome maintenance helicases.
- Regulation of minichromosome maintenance helicase activity by Cdc6.
- The diverse spectrum of sliding clamp interacting proteins.
- Archaea: an archetype for replication initiation studies?
- Structural lessons in DNA replication from the third domain of life.
- Crystal structure of YbaB from Haemophilus influenzae (HI0442), a protein of unknown function coexpressed with the recombinational DNA repair protein RecR.
- The HI0073/HI0074 protein pair from Haemophilus influenzae is a member of a new nucleotidyltransferase family: structure, sequence analyses, and solution studies.
- DNA replication in the third domain (of life).
- The Methanobacterium thermoautotrophicum MCM protein can form heptameric rings.
- The replication origin of archaea is finally revealed.
- Characterization of an ATP-dependent DNA ligase from the thermophilic archaeon Methanobacterium thermoautotrophicum.
- Proliferating cell nuclear antigen as the cell cycle sensor for an HLA-derived peptide blocking T cell proliferation.
- Fidelity of eucaryotic DNA polymerase delta holoenzyme from Schizosaccharomyces pombe.
- A unique organization of the protein subunits of the DNA polymerase clamp loader in the archaeon Methanobacterium thermoautotrophicum deltaH.
- Structure and activity associated with multiple forms of Schizosaccharomyces pombe DNA polymerase delta.
- The single minichromosome maintenance protein of Methanobacterium thermoautotrophicum DeltaH contains DNA helicase activity.
- Multiple competition reactions for RPA order the assembly of the DNA polymerase delta holoenzyme.
- Isolation and characterization of a split B-type DNA polymerase from the archaeon Methanobacterium thermoautotrophicum deltaH.
- The C-terminal region of Schizosaccaromyces pombe proliferating cell nuclear antigen is essential for DNA polymerase activity.
- Studies on the interactions between human replication factor C and human proliferating cell nuclear antigen.
- Trading places on DNA--a three-point switch underlies primer handoff from primase to the replicative DNA polymerase.
- The internal workings of a DNA polymerase clamp-loading machine.
- DNA polymerase delta isolated from Schizosaccharomyces pombe contains five subunits.
- PCNA: structure, functions and interactions.
- The influence of the proliferating cell nuclear antigen-interacting domain of p21(CIP1) on DNA synthesis catalyzed by the human and Saccharomyces cerevisiae polymerase delta holoenzymes.
- Dynamics of loading the beta sliding clamp of DNA polymerase III onto DNA.
- Structure of the C-terminal region of p21(WAF1/CIP1) complexed with human PCNA.
- Degradation of ancient DNA.
- Clamp loading, unloading and intrinsic stability of the PCNA, beta and gp45 sliding clamps of human, E. coli and T4 replicases.
- Escherichia coli expression vectors containing a protein kinase recognition motif, His6-tag and hemagglutinin epitope.
- Protein structure. Why have six-fold symmetry?
- Structural and functional similarities of prokaryotic and eukaryotic DNA polymerase sliding clamps.
- Embryonic PCNA: a missing link?
- Radiolabeling of proteins for biochemical studies.
- DNA polymerase III holoenzyme: structure and function of a chromosomal replicating machine.