Kyle Anderson

Hudgens Group

Contact

Email: kwa5025@umd.edu

Call: (240) 314-6285

Education

  • NIST-NRC Postdoctoral Research Associate, 2016
  • PhD, Biochemistry, University of Maryland College Park, 2015
  • BS, Biochemistry and Molecular Biology, Pennsylvania State University, 2010

Profile

Kyle Anderson is a NIST research chemist located at IBBR. His current focus is hydrogen/deuterium exchange mass spectrometry (HDX-MS) for characterization of biopharmaceuticals and vaccines. Additionally, he is involved in method development and measurement improvement of HDX-MS. He received the U.S. Department of Commerce Silver Medal for developing and promoting adoption of methods to determine protein structure that increase patient access to biosimilar drugs. He is President of the Washington Chromatography Discussion Group and a member of the International Society of HDX-MS.

I am accepting applications for NIST-NRC postdoctoral associates in HDX-MS. Application deadlines are Feb 1 and Aug 1. Contact Kyle Anderson before applying. View Job Posting.

 

CURRENT RESEARCH

Chromatography at -30 °C for reduced back-exchange, reduced carryover, and improved dynamic range for HDX-MS

Characterization of protein vaccines in formulation by HDX-MS

Characterization of drug-protein interactions by HDX-MS

Publications
2021
HDX-MS and MD Simulations Provide Evidence for Stabilization of the IgG1-FcγRIa (CD64a) Immune Complex Through Intermolecular Glycoprotein Bonds.
Copper-binding anticancer peptides from the piscidin family: an expanded mechanism that encompasses physical and chemical bilayer disruption.
2020
Conformational gating, dynamics and allostery in human monoacylglycerol lipase.
2019
Interlaboratory Comparison of Hydrogen-Deuterium Exchange Mass Spectrometry Measurements of the Fab Fragment of NISTmAb.
Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS) Centroid Data Measured between 3.6 °C and 25.4 °C for the Fab Fragment of NISTmAb.
2018
Automated Removal of Phospholipids from Membrane Proteins for H/D Exchange Mass Spectrometry Workflows.
2017
Transcriptional and post-translational changes in the brain of mice deficient in cholesterol removal mediated by cytochrome P450 46A1 (CYP46A1).
Assessment of Extracellular Vesicles Purity Using Proteomic Standards.
In vitro cytochrome P450 46A1 (CYP46A1) activation by neuroactive compounds.
Conformational Changes in Active and Inactive States of Human PP2Cα Characterized by Hydrogen/Deuterium Exchange-Mass Spectrometry.
Cytochrome P450 27A1 Deficiency and Regional Differences in Brain Sterol Metabolism Cause Preferential Cholestanol Accumulation in the Cerebellum.
A new approach to quantification of mAb aggregates using peptide affinity probes.
2016
Mapping of the Allosteric Site in Cholesterol Hydroxylase CYP46A1 for Efavirenz, a Drug That Stimulates Enzyme Activity.
2015
Quantification of Borrelia burgdorferi Membrane Proteins in Human Serum: A New Concept for Detection of Bacterial Infection.
Histone post-translational modifications in frontal cortex from human donors with Alzheimer's disease.
Histone H3 Ser57 and Thr58 phosphorylation in the brain of 5XFAD mice.
Quantification of histone deacetylase isoforms in human frontal cortex, human retina, and mouse brain.
2014
Natural flanking sequences for peptides included in a quantification concatamer internal standard.
2012
Subsecond absolute quantitation of amine metabolites using isobaric tags for discovery of pathway activation in mammalian cells.